The last mention of the Holy Ghost is in the book of Jude.

Source: Jude 20

Who did Paul say seals us as believers in God?

Paul said several times it is the Holy Spirit which seals us.

Source: 2^nd Corinthians 1:22, Ephesians 1:13 also 4:30

Jesus said this sin involving the Holy Spirit is unforgivable. What is the sin?

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The unforgivable sin is blasphemy against the Holy Spirit. This is attributing the Holy Spirit’s actions to the devil.

Source: Matthew 12:31-32 Luke 12:10

What animal form did the Spirit take when he descended on Jesus at his baptism?

The Holy Spirit descended on Jesus in the form of a dove. Only Luke’s gospel omits this.

Source: Matthew 3:16, Mark 1:10, John 1:32

When was John the Baptist filled with the Holy Spirit?

John was filled with the Holy Spirit while in his Mother’s womb. Mary’s visit to Elizabeth confirmed this.

Source: Luke 1:15 verses 40-44 give John’s recognition of Jesus

What was the first appearance of the Holy Spirit in the New Testament?